Because each proton resonance has the same response factor (K) the relative quantity of each signal can be easily determined by comparative integration. The premise of qNMR is that the observed signal intensity is proportional to the number of nuclei that give rise to that particular resonance. (2) if the sample is fully soluble, every component can be observed and has the same response factor.ĭepending on experiment conditions and the external magnetic field strength (Bo), quantitative inaccuracies can be as low as 2.0% (not too shabby!) and can be validated with typical analytical parameters (e.g., precision, accuracy, linearity, reproducibility). Although not a particularly sensitive technique, NMR (my technique of choice!) is ideal for quantification because: Since the early 1960s the efficacy of NMR in quantitative measurements (qNMR) has been explored.
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